General |
Detection modes |
Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa®, Luminescence, UV-Visible Absorbance |
Read methods |
End point, kinetic, spectral scanning, well-area scanning |
Microplate types |
6- to 1536-well plates (luminescence 1- to 384-well plates) PCR plates |
Other labware supported |
Take3™ Micro-Volume Plate |
Temperature control |
4-Zone™ incubation to 65 °C; variation +0.2 °C at 37 °C |
Shaking |
Linear |
Software |
Gen5™ Data Analysis Software |
Automation |
BioStack™ and 3rd party automation compatible |
AlphaScreen/AlphaLISA |
Light source |
Tungsten halogen |
Sensitivity |
100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate |
Dynamic range |
>6 decades |
Reading speed |
2 minutes (96-well plate) |
Detection system |
PMT |
Absorbance |
Light source |
Xenon flash |
Wavelength selection |
Monochromator |
Wavelength range |
200 - 999 nm, 1 nm increment |
Bandwidth |
2.4 nm |
Dynamic range |
0 - 4.0 OD |
Resolution |
0.0001 OD |
Pathlength correction |
Yes |
Monochromator wavelength accuracy |
+2 nm |
Monochromator wavelength repeatability |
+0.2 nm |
OD accuracy |
<1% at 2 OD <3% at 3.0 OD |
OD linearity |
<1% from 0 to 3.0 OD |
OD repeatability |
<0.5% at 2.0 OD |
Stray light |
0.03% at 230 nm |
Reading speed |
96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds |
Fluorescence Intensity |
Light source |
Tungsten halogen High energy DPR Xenon flash (optional) |
Detector |
PMT |
Wavelength selection |
Deep blocking bandpass filters/dichroic mirrors |
Wavelength range |
300 - 700 nm with tungsten lamp (850 nm option) 200 - 700 nm with xenon lamp (850 nm option) |
Dynamic range |
>6 decades |
Sensitivity |
Top: Fluorescein 1 pM (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate)
Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) |
Bandwidth |
Filter dependent |
Reading speed |
96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds |
Luminescence |
Wavelength range |
300 - 700 nm |
Dynamic range |
>6 decades |
Sensitivity |
10 amol ATP (flash) 100 amol ATP (glow) |
Detection system |
PMT |
Fluorescence Polarization |
Light source |
Tungsten halogen High energy DPR Xenon flash (optional) |
Wavelength selection |
Deep blocking filters/dichroic mirrors |
Wavelength range |
400 - 700 nm (320 - 850 nm option) |
Sensitivity |
3 mP at 1 nM fluorescein |
Time-Resolved Fluorescence |
Light source |
High Energy DPR Xenon flash |
Wavelength selection |
Deep blocking filters/mirrors |
Wavelength range |
200 - 700 nm (850 nm option) |
Sensitivity |
Europium 60 fM (12 amol/well 96-well plate; 6 amol/well 384-well plate) |
Reagent Dispensers |
Number |
2 syringe pumps |
Supported labware |
6- to 96-well microplates |
Dead volume |
1 mL, 100 µL with back flush |
Dispense volume |
5 - 1000 µL in 1 µL increment |
Dispense accuracy |
+1 µL or 2% |
Dispense precision |
<2% at 50 - 200 µL |
Physical Characteristics |
Power |
250 Watts max. |
Dimensions |
17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm) |
Weight |
60 lbs (27 kg) |