This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Poly (ADP-Ribose). Poly (ADP-Ribose) in the sample or standard competes with a fixed amount of biotin-labeled Poly (ADP-Ribose) for sites on a pre-coated antibody specific to Poly (ADP-Ribose). Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Poly (ADP-Ribose) in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
