Антитела Anti-MGMT antibody [EPR4397], кроличьи моноклональные RabMab

Overview

Product nameAnti-MGMT antibody [EPR4397]
See all MGMT primary antibodies
Description
Rabbit monoclonal [EPR4397] to MGMT
Tested applicationsWB, IHC-Pmore details
Species reactivity
Reacts with: Human
Immunogen
A synthetic peptide corresponding to residues in Human MGMT.
Positive control
- WB: MCF7, Jurkat, HeLa, and HT29 cell lysates IHC-P: Hman clear cell carcinoma and spleen tissues
General notes
This product is a recombinant rabbit monoclonal antibody.

Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

FormLiquid
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
PurityTissue culture supernatant
ClonalityMonoclonal
Clone numberEPR4397
IsotypeIgG
Research areas

Applications

Our Abpromise guarantee covers the use of ab108630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IHC-P		1/250 - 1/500. Antigen retrieval is recommended.

Application notesIs unsuitable for Flow Cyt,ICC or IP.

Target

FunctionInvolved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Sequence similaritiesBelongs to the MGMT family.
Cellular localizationNucleus.
Information by UniProt
Database links
- Entrez Gene: 4255 Human
- Omim: 156569 Human
- SwissProt: P16455 Human
- Unigene: 501522 Human
Alternative names
- 6 O methylguanine DNA methyltransferase antibody
- 6-O-methylguanine-DNA methyltransferase antibody
- Agat antibody
see all

Anti-MGMT antibody [EPR4397] images

Western blot - Anti-MGMT antibody [EPR4397] (ab108630)

Predicted band size : 22 kDa

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MGMT knockout HAP1 cell lysate (20 µg)

Lane 3: MCF7 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108630 observed at 22 kDa. Red - loading control,ab8245, observed at 37 kDa.
ab108630 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - MGMT antibody [EPR4397] (ab108630)

All lanes : Anti-MGMT antibody [EPR4397] (ab108630) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat ell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HT29 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size : 22 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MGMT antibody [EPR4397] (ab108630)

ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MGMT antibody [EPR4397] (ab108630)

ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.

References for Anti-MGMT antibody [EPR4397] (ab108630)

Информация для заказа

Область использования: Антитела Abcam	Производство:	Abcam
	Метод:	Иммуногистохимия
	Объем:	100 мкл
	Кат. номер:	ab108630
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Антитела Anti-MGMT antibody [EPR4397], кроличьи моноклональные RabMab. Примечание: Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. Дополнительная информация (на английском).