Brilliant Violet 421™ anti-human CD11b AntibodyDescription
CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.
Product Details
- Isotype Control
- Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- β2 integrins from granulocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
- Violet Laser (405 nm)
- Application Notes
-
LM2 does not block clone ICRF44 indicating a unique epitope.
- RRID
- AB_2750257 (BioLegend Cat. No. 393113)
AB_2750258 (BioLegend Cat. No. 393114)
- Structure
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
- Distribution
-
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
- Function
- Adhesion, phagocytosis, chemotaxis, neutrophil activation
- Ligand/Receptor
- ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
- Cell Type
- Granulocytes, Monocytes, Macrophages, Dendritic cells, NK cells, T cells, B cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
- Miller LJ, et al. 1986. J Immunol. 137(9):2891.
- Gene ID
- 3684 View all products for this Gene ID
- UniProt
- View information about CD11b on UniProt.org
Информация для заказа| Область использования: | Производство: | Biolegend | | Метод: | | | Объем: | 100 | | Кат. номер: | 393114 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Brilliant Violet 421™ anti-human CD11b Antibody.
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