Rabbit anti-human Eukaryotic translation initiation factor 3 subunit I polyclonal Antibody;FITC conjugated

Immunogen: Recombinant human Eukaryotic translation initiation factor 3 subunit I protein
Raised in: Rabbit
Species Reactivity: Human
Tested applications: ELISA, WB, IHC
Application notes:
Relevance: EIF3S2 is the largest of the EIFs. It consists of at least 10 nonidentical subunits in mammals. In S. cerevisiae the p39 subunit contains WD repeats; these are thought to mediate protein-protein interactions. The p39 protein appears to be essential for maintaining the integrity of the yeast EIF3 complex. The mammalian EIF3-p36 subunit is homologous to yeast p39.
Clonality: Polyclonal
Isotype: IgG
Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
Conjugate: FITC conjugated
Stroage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form: Liquid
Stroage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
References: [1] "Conservation and diversity of eukaryotic translation initiation factor eIF3."Asano K., Kinzy T.G., Merrick W.C., Hershey J.W.B.J. Biol. Chem. 272:1101-1109(1997) [2] "A WD-domain protein that is associated with and phosphorylated by the type II TGF-beta receptor."Chen R.H., Miettinen P.J., Maruka E.M., Choy L., Derynck R.Nature 377:548-552(1995) [3] "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004)
Images:

Western blot

All lanes : Eukaryotic translation initiation factor 3, subunit 2 beta antibody at at 2 µg/ml

Lane 1 : EC109whole cell lysate
Lane 2 : 293T whole cell lysate

Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution

Predicted band size : 36 kDa
Observed band size : 80 kDa

Why is the actual band size different from the predicted?

Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

· post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein

· post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases

· splice variants - alternative splicing may create different sized proteins from the same gene

· relative charge - the composition of amino acids (charged vs non-charged)

multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.

Alias : EIF3S2, TRIP1,EIF3I,Eukaryotic translation initiation factor 3 subunit 2,TGF-beta receptor-interacting protein 1,TRIP-1,eIF-3-beta,eIF3 p36,eIF3i.

Информация для заказа

Область использования: Поликлональные антитела Cusabio	Производство:	Cusabio
	Метод:
	Объем:	100 мкг
	Кат. номер:	CSB-PA03105B0Rb
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Rabbit anti-human Eukaryotic translation initiation factor 3 subunit I polyclonal Antibody;FITC conjugated. Примечание: дополнительная информация (на английском языке).