Объем | 100 мкг |
Синонимы | Tumor necrosis factor receptor superfamily member 11A (Osteoclast differentiation factor receptor) (ODFR) (Receptor activator of NF-KB) (CD antigen CD265), TNFRSF11A, RANK |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9Y6Q6 |
Иммуноген | Recombinant Human Tumor necrosis factor receptor superfamily member 11A protein (28-202AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200 |
Примечание | Receptor for TNFSF11/RANKL/TRANCE/OPGL, essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Tumor necrosis factor receptor superfamily member 11A protein |
Области исследований | Cardiovascular, Immunology, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Tumor necrosis factor receptor superfamily member 11A antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 67, 33, 37, 29, 57, 65 kDa
Observed band size: 67 kDa
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Immunofluorescence staining of PC-3 cells with CSB-PA08467A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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