| Объем | 100 мкг |
| Синонимы | Catenin beta-1 (Beta-catenin), CTNNB1, CTNNB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P35222 |
| Иммуноген | Recombinant Human Catenin beta-1 protein (2-781AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Involved in signal transduction through the Wnt pathway. Nuclear beta-catenin it involved in transcriptional regulation by association with transcription factors of the TCF/LEF family. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Catenin beta-1 protein |
| Области исследований | Neuroscience, Cancer, Cardiovascular, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00174A0Rb at dilution of 1:100
 |
Immunofluorescence staining of HepG2 cells with CSB-PA00174A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
 |
Immunoprecipitating CTNNB1 in HT29 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA00174A0Rb in HT29 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA00174A0Rb (8µg) + HT29 whole cell lysate (500µg)
Lane 3: HT29 whole cell lysate (20µg)
 |
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate
All lanes: CTNNB1 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa
 |
IHC image of CSB-PA00174A0Rb diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 |
Immunofluorescence staining of Hela cells with CSB-PA00174A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
 |
| | |
Наименование: Антитела Rabbit anti-human Catenin beta-1 polyclonal Antibody / Rabbit anti-human Catenin beta-1 polyclonal Antibody.