| Объем | 100 мкг |
| Синонимы | Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS) [Cleaved into: Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed], YARS |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P54577 |
| Иммуноген | Recombinant Human Tyrosyl-tRNA synthetase, cytoplasmic protein (2-528AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Tyrosyl-tRNA synthetase, cytoplasmic antibody at 2µg/ml + 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00465A0Rb at dilution of 1:50
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Immunofluorescence staining of HepG2 cells with CSB-PA00465A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: Антитела Rabbit anti-human Tyrosyl-tRNA synthetase, cytoplasmic polyclonal Antibody / Rabbit anti-human Tyrosyl-tRNA synthetase, cytoplasmic polyclonal Antibody.