Объем | 100 мкг |
Синонимы | ATP synthase subunit delta, mitochondrial (F-ATPase delta subunit), ATP5D |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P30049 |
Иммуноген | Recombinant Human ATP synthase subunit delta, mitochondrial protein (44-553AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATP synthase subunit delta, mitochondrial antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 36 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00635A0Rb at dilution of 1:50
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA00635A0Rb at dilution of 1:50
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