Антитела Rabbit anti-human Fibroblast growth factor 21 polyclonal Antibody

Rabbit anti-human Fibroblast growth factor 21 polyclonal Antibody

Immunogen: Recombinant human Fibroblast growth factor 21 protein
Raised in: Rabbit
Species Reactivity: Human
Tested applications: ELISA, WB, IHC
Application notes:
Relevance: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
Clonality: Polyclonal
Isotype: IgG
Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
Conjugate: Non-conjugated
Stroage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form: Liquid
Stroage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
References: [1] "Identification of a novel FGF, FGF-21, preferentially expressed in the liver."Nishimura T., Nakatake Y., Konishi M., Itoh N.Biochim. Biophys. Acta 1492:203-206(2000) [2] "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.Genome Res. 13:2265-2270(2003) [3] "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004)
Images:

Western blot

All lanes : Fibroblast growth factor 21 1 antibody at at 2 µg/ml

Lane 1 : 293T whole cell lysate
Lane 2 : EC109 whole cell lysate

Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution

Predicted band size : 23 kDa

Observed band size: 27 kDa

Additional bands at : 38kDa. 50 kDa.85 kDa.115 kDa.We are unsure as to the identity of these extra bands.

Why is the actual band size different from the predicted?

Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

· post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein

· post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases

· splice variants - alternative splicing may create different sized proteins from the same gene

· relative charge - the composition of amino acids (charged vs non-charged)

multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands

Alias : UNQ3115/PRO10196,FGF21,FGF-21.

Информация для заказа

Область использования: Поликлональные антитела Cusabio	Производство:	Cusabio
	Метод:
	Объем:	100 мкг
	Кат. номер:	CSB-PA06407A0Rb
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Антитела Rabbit anti-human Fibroblast growth factor 21 polyclonal Antibody / Rabbit anti-human Fibroblast growth factor 21 polyclonal Antibody. Примечание: дополнительная информация (на английском языке).