| Объем | 100 мкг |
| Синонимы | Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (PP2A-beta) (EC 3.1.3.16), PPP2CB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P62714 |
| Иммуноген | Recombinant Human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform protein (1-309AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform protein |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2µg/ml + EC109 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00985A0Rb at dilution of 1:50
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Immunofluorescent analysis of Hela cells using CSB-PA00985A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00985A0Rb at dilution of 1:100
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Наименование: Антитела Rabbit anti-human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform polyclonal Antibody / Rabbit anti-human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform polyclonal Antibody.