Объем | 100 мкг |
Синонимы | NADH-cytochrome b5 reductase 3 (B5R) (Cytochrome b5 reductase) (EC 1.6.2.2) (Diaphorase-1) [Cleaved into: NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form], CYB5R3, DIA1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P00387 |
Иммуноген | Recombinant Human NADH-cytochrome b5 reductase 3 protein (1-301AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | DIAPH1 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). In hearing it may play a role in the regulation of actin polymerization in hair cells. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | NADH-cytochrome b5 reductase 3 protein |
Области исследований | Cancer, Cardiovascular, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NADH-cytochrome b5 reductase 3 antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 35, 32, 39 kDa
Observed band size: 32 kDa
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Immunohistochemistry of paraffin-embedded human lungs tissue using CSB-PA02655A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA02655A0Rb at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA02655A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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