| Объем | 100 мкг |
| Синонимы | Heat shock protein 75 kDa, mitochondrial (HSP 75) (TNFR-associated protein 1) (Tumor necrosis factor type 1 receptor-associated protein) (TRAP-1), TRAP1, HSP75 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q12931 |
| Иммуноген | Recombinant Human Heat shock protein 75 kDa, mitochondrial protein (60-308AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Chaperone that expresses an ATPase activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Heat shock protein 75 kDa, mitochondrial protein |
| Области исследований | Cancer, Cell biology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Heat shock protein 75 kDa, mitochondrial antibody at 2µg/ml + EC109whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 81, 75 kDa
Observed band size: 75 kDa
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Immunofluorescent analysis of MCF-7 cells using CSB-PA03604A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Mouse heart tissue
All lanes: TRAP1 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 81, 75 kDa
Observed band size: 81 kDa
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Immunofluorescent analysis of Hela cells using CSB-PA03604A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating TRAP1 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA03604A0Rb in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA03604A0Rb (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
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Наименование: Антитела Rabbit anti-human Heat shock protein 75 kDa, mitochondrial polyclonal Antibody / Rabbit anti-human Heat shock protein 75 kDa, mitochondrial polyclonal Antibody.