| Объем | 100 мкг |
| Синонимы | Transcription intermediary factor 1-beta (TIF1-beta) (E3 SUMO-protein ligase TRIM28) (EC 2.3.2.27) (KRAB-associated protein 1) (KAP-1) (KRAB-interacting protein 1) (KRIP-1) (Nuclear corepressor KAP-1) (RING finger protein 96) (RING-type E3 ubiquitin transferase TIF1-beta) (Tripartite motif-containing protein 28), TRIM28, KAP1 RNF96 TIF1B |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13263 |
| Иммуноген | Recombinant Human Transcription intermediary factor 1-beta protein (22-291AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Forms a complex with a KRAB-domain transcription factor and increases the efficiency of KRAB-mediated repression. Silences transcription through an interaction with HP1 proteins. Acts as a corepressor of transcription for the KRAB zinc finger proteins and as a moderator of the repression activity. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of the Alpha-1-acid glycoprotein gene. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Transcription intermediary factor 1-beta protein |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate
All lanes: TRIM28 antibody at 1.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 80 kDa
Observed band size: 89 kDa
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA05254A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA05254A0Rb at dilution of 1:100
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Immunoprecipitating TRIM28 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA05254A0Rb in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA05254A0Rb (6µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: Антитела Rabbit anti-human Transcription intermediary factor 1-beta polyclonal Antibody / Rabbit anti-human Transcription intermediary factor 1-beta polyclonal Antibody.