| Объем | 100 мкг |
| Синонимы | B-cell receptor-associated protein 31 (BCR-associated protein 31) (Bap31) (6C6-AG tumor-associated antigen) (Protein CDM) (p28), BCAP31, BAP31 DXS1357E |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P51572 |
| Иммуноген | Recombinant Human B-cell receptor-associated protein 31 protein (2-243AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:1000-1:2000, IF:1:50-1:500, IP:1:200-1:2000 |
| Примечание | May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | B-cell receptor-associated protein 31 protein |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat liver tissue, Rat brain tissue, Mouse liver tissue, Mouse brain tissue
All lanes: BCAP31 antibody at 4.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28, 35 kDa
Observed band size: 28 kDa
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IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescent analysis of Hela cells using CSB-PA03425A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating BCAP31 in Mouse liver whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA03425A0Rb in Mouse liver whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA03425A0Rb (6µg) + Mouse liver whole cell lysate (500µg)
Lane 3: Mouse liver whole cell lysate (10µg)
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Наименование: Антитела Rabbit anti-human B-cell receptor-associated protein 31 polyclonal Antibody / Rabbit anti-human B-cell receptor-associated protein 31 polyclonal Antibody.