| Объем | 100 мкг |
| Синонимы | HCLS1-associated protein X-1 (HS1-associating protein X-1) (HAX-1) (HS1-binding protein 1) (HSP1BP-1), HAX1, HS1BP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O00165 |
| Иммуноген | Recombinant Human HCLS1-associated protein X-1 protein (2-250AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
| Примечание | Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | HCLS1-associated protein X-1 protein |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue, Rat brain tissue
All lanes: HAX1 antibody at 1.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32, 33, 29, 15, 27, 22 kDa
Observed band size: 32 kDa
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IHC image of CSB-PA03515A0Rb diluted at 1:150 and staining in paraffin-embedded human stomach cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescent analysis of Hela cells using CSB-PA03515A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: Антитела Rabbit anti-human HCLS1-associated protein X-1 polyclonal Antibody / Rabbit anti-human HCLS1-associated protein X-1 polyclonal Antibody.