Объем | 100 мкг |
Синонимы | High mobility group protein B1 (High mobility group protein 1) (HMG-1), HMGB1, HMG1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P09429 |
Иммуноген | Recombinant Human High mobility group protein B1 protein (8-179AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | High mobility group protein B1 protein |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA01604A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA01604A0Rb at dilution of 1:100
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Western blot
All lanes: HMGB1 antibody at 10 µg/ml
Lane 1: Raji whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 24, 40 kDa
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Western Blot
Positive WB detected in: Mouse brain
All lanes: HMGB1 antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
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Western Blot
Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, Raji whole cell lysate
All lanes: HMGB1 antibody at 1:2500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
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