Антитела Rabbit anti- human V-type proton ATPase subunit G 1 polyclonal Antibody,Biotin conjugated

Rabbit anti- human V-type proton ATPase subunit G 1 polyclonal Antibody,Biotin conjugated

Immunogen: Recombinant human V-type proton ATPase subunit G 1 protein
Raised in: Rabbit
Species Reactivity: Human,Mouse, Rat
Tested applications: ELISA, WB, IHC
Application notes:
Relevance: This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
Clonality: Polyclonal
Isotype: IgG
Purity: Caprylic Acid Ammonium Sulfate Precipitation
Conjugate: Biotin
Stroage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form: Liquid
Stroage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
References: [1] "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [2] "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004)
Images:

Western blot

V-type proton ATPase subunit G 1 Antibody at 2 µg/ml + 293T whole cell lysate at 20 µg

Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution

Predicted band size : 13 kDa
Observed band size : 50 kDa

Why is the actual band size different from the predicted?

Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

· post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein

· post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases

· splice variants - alternative splicing may create different sized proteins from the same gene

· relative charge - the composition of amino acids (charged vs non-charged)

multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands

Alias : .

Информация для заказа

Область использования: Поликлональные антитела Cusabio	Производство:	Cusabio
	Метод:
	Объем:	100 мкг
	Кат. номер:	CSB-PA00134D0Rb
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Антитела Rabbit anti- human V-type proton ATPase subunit G 1 polyclonal Antibody,Biotin conjugated / Rabbit anti- human V-type proton ATPase subunit G 1 polyclonal Antibody,Biotin conjugated. Примечание: дополнительная информация (на английском языке).