Rabbit Anti Human PRKCA PolyClonal AntibodyСпецификация| Объем | 100 мкл (100 мкг) | | Синонимы | PKCA, AAG6, PKCA, PRKACA, PKC-alpha, MGC129900, MGC129901, PRKCA | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P17252 | | Иммуноген | C term -peptide of human PRKCA | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:2000, IHC:1:50-1:200 | | Примечание | Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation at Thr500 in the activation loop, the autophosphorylation site at Thr641, and at carboxy-terminal hydrophobic site Ser660 occurs in vivo. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Either the enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity purification | | Области исследований | Cancer,Cardiovascular,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts of Jurkat and K562 cells, using PKC? antibody.
 | Immunohistochemistry of paraffin-embedded Pancreas cancer using PKCA Antibody.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | | | Объем: | 100 мкл (100 мкг) | | Кат. номер: | CSB-PA018699KA01HU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Rabbit Anti Human PRKCA PolyClonal Antibody.
Примечание: дополнительная информация (на английском языке). |
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