Объем | 100 мкг |
Синонимы | Thymidylate synthase (TS) (TSase) (EC 2.1.1.45), TYMS, TS |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P04818 |
Иммуноген | Recombinant Human Thymidylate synthase protein (2-54AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
Примечание | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Thymidylate synthase |
Области исследований | Epigenetics and Nuclear Signaling, Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate
All lanes: TYMS antibody at 2.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 32, 27 kDa
Observed band size: 36 kDa
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Immunofluorescent analysis of Hela cells using CSB-PA025393LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating TYMS in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA025393LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA025393LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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