Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image :
Western blot All lanes: SMAD4 antibody at 12 ug/ml Lane 1?: SH-SY5Y whole cell lysate Lane 2?: 3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60, 30 kDa
Western blot All lanes: SMAD4 antibody at 3 ug/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA619768LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA619768LA01HU at dilution of 1:100
Immunoprecipitating SMAD4 in Jurkat whole cell lysate Lane 1: Rabbit control IgG(1ug)instead of CSB-PA619768LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA619768LA01HU(8ug)+ Jurkat whole cell lysate(500ug) Lane 3: Jurkat whole cell lysate (10ug)
Immunogen :
Recombinant human Mothers against decapentaplegic homolog 4 protein (27-195AA)
In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
