TP53 Antibody (Р-53)Спецификация| Объем | 100 мкг | | Синонимы | Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53), TP53, P53 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P04637 | | Иммуноген | Recombinant Human Cellular tumor antigen p53 protein (2-393AA) | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, ChIP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 | | Примечание | Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediatedapoptosis. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot
All lanes: Cellular tumor antigen p53 antibody at 2µg/ml
Lane 1: A431 whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: MM231 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 44 kDa
 | Western Blot
Positive WB detected in: Mouse kidney
All lanes: p53 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 44 kDa
 | Western Blot
Positive WB detected in: HEK293 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse kidney tissue
All lanes: p53 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 44 kDa
 | Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA07889A0Rb at dilution of 1:100
 | Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-p53 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP07889HU) against the P21 promoter.
 | Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: TP53 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 44 kDa
 | Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA07889A0Rb at dilution of 1:100
 | Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA07889A0Rb at dilution of 1:100
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Иммуногистохимия | | Объем: | 100 мкг | | Кат. номер: | CSB-PA07889A0Rb | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: TP53 Antibody (Р-53).
Примечание: дополнительная информация (на английском языке). |
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