| Объем | 100 мкг |
| Синонимы | AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1), AAK1, KIAA1048 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q2M2I8 |
| Иммуноген | Recombinant Human AP2-associated protein kinase 1 protein (274-465AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | AP2-associated protein kinase 1 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse kidney tissue
All lanes: AAK1 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 104, 94 kDa
Observed band size: 104 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA643571LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA643571LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: AAK1 Antibody.