AAK1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | AP2-associated protein kinase 1, EC 2.7.11.1, Adaptor-associated kinase 1, AAK1, KIAA1048 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q2M2I8 | | Иммуноген | Synthesized peptide derived from internal of human AAK1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:3000 | | Примечание | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.<br/><br/>Kikuno R., DNA Res. 6:197-205(1999).<br/>Hillier L.W., Nature 434:724-731(2005).<br/>Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from COS7 cells, using AAK1 antibody.
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Наименование: AAK1 Antibody.