2-hydroxyisobutyryl-HIST1H4A (K12) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, AND, HIST4H4 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P62805 | | Иммуноген | Peptide sequence around site of 2-hydroxyisobutyryl-Lys (12) derived from human Histone H4 | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, ICC, IF, IP, ChIP, Recommended dilution: WB:1:100-1:1000, ICC:1:20-1:200, IF:1:10-1:100, IP:1:200-1:2000 | | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Абревеатура | Histone H4 | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Detected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H4A antibody at 1.25µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
 | Immunocytochemistry analysis of CSB-PA010429PA12hibHU diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010429PA12hibHU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
 | Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010429PA12hibHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h).
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010429PA12hibHU (3µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg)
Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
 | Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5?g anti-HIST1H4A (CSB-PA010429PA12hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA010429PA12hibHU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: 2-hydroxyisobutyryl-HIST1H4A (K12) Antibody.
Примечание: дополнительная информация (на английском языке). |
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