2-hydroxyisobutyryl-HIST1H3A (K56) Antibody

Спецификация

Объем	50 мкл
Синонимы	Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ
Клональность	Polyclonal Antibody
Организм	Human
uniprot	P68431
Иммуноген	Peptide sequence around site of 2-hydroxyisobutyryl-Lys(56) derived from Human Histone H3
Источник	Rabbit
Видовая специфичность	Human
Применение	ELISA, WB, IF, Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200
Примечание	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Клональность1	Polyclonal
Изотип	IgG
Коньюгат	Non-conjugated
Буффер	Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Форма	Liquid
Хранение	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки	Antigen Affinity Purified
Абревеатура	Histone H3.1
Области исследований	Epigenetics and Nuclear Signaling
Ссылка на страницу на сайте производителя	ссылка
Immunofluorescence staining of Hela cells with CSB-PA010418OA56hibHU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate (treated by 30mM sodium butyrate for 4h) All lanes: HIST1H3A antibody at 0.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa

Информация для заказа

Область использования: Поликлональные антитела Cusabio	Производство:	Cusabio
	Метод:	Антитела
	Объем:	50 мкл
	Кат. номер:	CSB-PA010418OA56hibHU
Цена (с НДС 20%):	по запросу	В корзину
Наименование: 2-hydroxyisobutyryl-HIST1H3A (K56) Antibody. Примечание: дополнительная информация (на английском языке).