| Объем | 100 мкл |
| Синонимы | Histone H2A.Z (H2A/z), H2AFZ, H2AZ |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P0C0S5 |
| Иммуноген | Peptide sequence around site of acetyl-Lys (7) derived from Human Histone H2A.Z. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, ICC, IF, IP, ChIP, Recommended dilution: ICC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2A.Z |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate (all treated by 30mM sodium butyrate for 4h)
All lanes: H2AFZ antibody at 0.48µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
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Immunofluorescence staining of Hela cells with CSB-PA010100OA07acHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (CSB-PA0101000A07acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Immunocytochemistry analysis of CSB-PA010100OA07acHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating H2AFZ in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010100OA07acHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h).
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010100OA07acHU (3µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)500µg)
Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
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Наименование: Acetyl-H2AFZ (K7) Antibody.