Объем | 100 мкл |
Синонимы | Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P16403 |
Иммуноген | Peptide sequence around site of Acetyl-Lys (62) derived from Human Histone H1.2. |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, ICC, IF, ChIP, Recommended dilution: ICC:1:20-1:200, IF:1:50-1:200 |
Примечание | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Histone H1.2 |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Immunocytochemistry analysis of Hela cells using CSB-PA010378PA62acHU at dilution of 1:100
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Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) tissue using CSB-PA010378PA62acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378PA62acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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