Acetyl-Histone H4 (K16) AntibodyСпецификация| Объем | 50 мкл | | Синонимы | Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, AND, HIST4H4 | | Тип антител | Recombinant Antibody | | Species | Human | | UniProt ID | P62805 | | Иммуноген | A synthesized peptide | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, ICC, IF, FC, Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200 | | Клональность | Monoclonal | | Изотип | Rabbit IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity-chromatography | | Области исследований | Epigenetics and Nuclear Signaling | | Аббревиатура | Histone H4 | | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in:293 whole cell lysate,HepG2 whole cell lysate,Mouse kidney tissue
All lanes:Acetyl-Histone H4 (K16) antibody at 1.65µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 KDa
Observed band size: 11 KDa
 | Immunocytochemistry analysis of CSB-RA010429A16acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010429A16acHU at 1:403,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
 | Overlay histogram showing Hela cells stained with CSB-RA010429A16acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 50 мкл | | Кат. номер: | CSB-RA010429A16acHU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Acetyl-Histone H4 (K16) Antibody.
Примечание: дополнительная информация (на английском языке). |
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