| Объем | 50 мкл |
| Синонимы | Histone H2A.Z (H2A/z), H2AFZ, H2AZ |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P0C0S5 |
| Иммуноген | Peptide sequence around site of acetyl-Lys (11) derived from Human Histone H2A.Z. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, ChIP, Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200 |
| Примечание | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2A.Z |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) tissue using CSB-PA010100PA11acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-H2AFZ (CSB-PA010100PA11acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Western Blot
Positive WB detected in: HepG2 whole cell lysate ( treated with 30mM sodium butyrate for 4h)
All lanes: H2AFZ antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
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Наименование: Acetyl-H2AFZ (K11) Antibody.