| Объем | 50 мкг |
| Синонимы | Actin, cytoplasmic 1 (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed], ACTB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P60709 |
| Иммуноген | Recombinant Human Actin, cytoplasmic 1 protein (102-375AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:50-1:200 |
| Примечание | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Actin, cytoplasmic 1 protein |
| Области исследований | Isotype/Loading Controls, Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ACTB antibody at 5µg/ml + Mouse spleen tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA01629A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA01629A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA01629A0Rb at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA01629A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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IHC image of CSB-PA01629A0Rb diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA01629A0Rb diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of HepG2 cells with CSB-PA01629A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: ACTB Antibody.