| Объем | 100 мкг |
| Клон ID | 3G5H8 |
| Синонимы | 6xHis, 6xHis, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag |
| Тип антител | Tag/Control Antibodies |
| Иммуноген | HHHHHH(6x His) synthetic peptide conjugate to KLH |
| Источник | Mouse |
| Применение | ELISA,WB,Recommended dilution:WB:1:500-1:5000 |
| Изотип | IgG2a |
| Коньюгат | Non-conjugated |
| Метод очистки | >95%,Protein G purified |
| Буффер | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Примечание | A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound. |
| Ссылка на страницу на сайте производителя | ссылка |
All lanes :Mouse Anti-6*his monoclonal antibody at 1ug/ml
Western blot analysis of 6*his Epitope Tag was performde by loading various amounts of lysates containing different HIS tagged
Recombinant proteins per well onto a 15% Tris-HCL polyacrylamide gel.
Line 1: FABP3 insect cell lysate
Line 2: TK1 insect cell lysate
Line 3: cagA insect cell lysate
Line 4: NGAL insect cell lysate
Line 5: IGFBP1 293T cell lysate
Line 6: TRY2 293T cell lysate
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All lanes : Recombinant DST Protein at 30ng/ml
Line 1: Mouse Anti-his monoclonal antibody at 1:64000
Line 2: Mouse Anti-his monoclonal antibody at 1:32000
Line 3: Mouse Anti-his monoclonal antibody at 1:16000
Line 4: Mouse Anti-his monoclonal antibody at 1:8000
Line 5: Mouse Anti-his monoclonal antibody at 1:4000
Observed band size: 35kd
Predicted band size: 35kd
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Наименование: 6xHis Monoclonal Antibody.