Acetyl-Histone H2A type 1-B/E (K9) AntibodyСпецификация| Объем | 50 мкл | | Синонимы | Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM, AND, HIST1H2AE, H2AFA | | Тип антител | Recombinant Antibody | | Species | Human | | UniProt ID | P04908 | | Иммуноген | A synthesized peptide | | Видовая специфичность | Human | | Применение | ELISA, ICC, IF, Recommended dilution: ICC:1:50-1:500, IF:1:30-1:200 | | Клональность | Monoclonal | | Изотип | Rabbit IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity-chromatography | | Области исследований | Epigenetics and Nuclear Signaling | | Аббревиатура | Histone H2A type 1-B/E | | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | | Ссылка на страницу на сайте производителя | ссылка | Immunocytochemistry analysis of CSB-RA010385A09acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010385A09acHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 50 мкл | | Кат. номер: | CSB-RA010385A09acHU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Acetyl-Histone H2A type 1-B/E (K9) Antibody.
Примечание: дополнительная информация (на английском языке). |
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