ADD1 (Ab-726) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | ADDA, Erythrocyte adducin alpha subunit, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P35611 | | Иммуноген | Peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described. <br/><br/>Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30, 102(35): 12407-12<br/>Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01, 332(2): 347-51<br/>Barkalow KL, et al. J Cell Biol 2003 May 12, 161(3): 557-70<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Tags & Cell Markers,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 and Hela cells using ADD1(Ab-726) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Ab-726) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Ab-726) Antibody.
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Наименование: ADD1 (Ab-726) Antibody.