| Объем | 100 мкг |
| Синонимы | Proline-rich AKT1 substrate 1 (40 kDa proline-rich AKT substrate), AKT1S1, PRAS40 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q96B36 |
| Иммуноген | Recombinant Human Proline-rich AKT1 substrate 1 protein (52-101AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:3000, IHC:1:20-1:200 |
| Примечание | Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Proline-rich AKT1 substrate 1 |
| Области исследований | Neuroscience, Cell biology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA836185LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA836185LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Rat stomach tissue
All lanes: AKT1S1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28, 14, 30 kDa
Observed band size: 28 kDa
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Наименование: AKT1S1 Antibody.