| Объем | 50 мкг |
| Синонимы | RAC-gamma serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-3) (Protein kinase B gamma) (PKB gamma) (RAC-PK-gamma) (STK-2), AKT3, PKBG |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9Y243 |
| Иммуноген | Recombinant Human RAC-gamma serine/threonine-protein kinase protein (1-164AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, IP, Recommended dilution: IP:1:200-1:2000 |
| Примечание | AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | RAC-gamma serine/threonine-protein kinase |
| Области исследований | Cancer, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunoprecipitating AKT3 in mouse brain whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA15929A0Rb in mouse brain whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA15929A0Rb (6µg) + Mouse brain whole cell lysate (500µg)
Lane 3: Mouse brain whole cell lysate (10µg)
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Наименование: AKT3 Antibody.