Объем | 100 мкг |
Синонимы | Protein AMBP [Cleaved into: Alpha-1-microglobulin (Protein HC) (Alpha-1 microglycoprotein) (Complex-forming glycoprotein heterogeneous in charge), Inter-alpha-trypsin inhibitor light chain (ITI-LC) (Bikunin) (EDC1) (HI-30) (Uronic-acid-rich protein), Trypstatin], AMBP, HCP ITIL |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P02760 |
Иммуноген | Recombinant Human Protein AMBP protein (20-203AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cancer, Cardiovascular, Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA001654YA01HU at dilution of 1:20
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Western blot
All lanes: AMBP antibody at 1.5µg/ml + Mouse liver tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate
All lanes: AMBP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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IHC image of CSB-PA001654YA01HU diluted at 1:150 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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