ALK AntibodyСпецификация| Объем | 100 мкл | | Синонимы | ALK tyrosine kinase receptor precursor, Anaplastic lymphoma kinase, CD246, EC 2.7.10.1, kinase ALK | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q9UM73 | | Иммуноген | Synthesized peptide derived from Internal of human ALK. | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, IHC, IF,IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.<br/><br/>Brunangelo Falini, Blood, Nov 1999, 94: 3509 - 3515. <br/>Philipp B Staber, Blood, Aug 2007, 10.1182/blood-2007-02-071258. <br/>Monika Kasprzycka, PNAS, Jun 2006, 103: 9964 - 9969. <br/>Ritsuro Suzuki, Blood, Nov 2000, 96: 2993 - 3000.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Neuroscience,Cancer,Tags & Cell Markers | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry analysis of paraffin-embedded human brain tissue using ALK antibody.
 | Immunofluorescence analysis of HeLa cells, using ALK antibody.
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Наименование: ALK Antibody.