| Объем | 50 мкг |
| Синонимы | Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35), ANXA1, ANX1 LPC1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04083 |
| Иммуноген | Recombinant Human Annexin A1 protein (5-346AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Annexin A1 protein |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Cadherin-12 antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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Western Blot
Positive WB detected in: PC-3 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: ANXA1 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
 |
Immunoprecipitating ANXA1 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA13457A0Rb in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA13457A0Rb (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
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Immunoprecipitating ANXA1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA13457A0Rb in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA13457A0Rb (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Immunofluorescence staining of Hela cells with CSB-PA13457A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
 |
Immunoprecipitating ANXA1 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA13457A0Rb in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA13457A0Rb (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
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Наименование: ANXA1 Antibody.