| Объем | 50 мкг |
| Синонимы | Muellerian-inhibiting factor (Anti-Muellerian hormone) (AMH) (Muellerian-inhibiting substance) (MIS), AMH, MIF |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P03971 |
| Иммуноген | Recombinant Human Muellerian-inhibiting factor protein (26-560AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:200-1:1000, IHC:1:100-1:1500, IF:1:50-1:200 |
| Примечание | This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Developmental biology, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: AMH antibody at 2µg/ml + Mouse small intestine tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA12639A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA12639A0Rb at dilution of 1:100
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Immunofluorescent analysis of MCF-7 cells using CSB-PA12639A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: AMH antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
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IHC image of CSB-PA12639A0Rb diluted at 1:1400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: AMH Antibody.