Объем | 50 мкг |
Синонимы | Apoptotic protease-activating factor 1 (APAF-1), APAF1, KIAA0413 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O14727 |
Иммуноген | Recombinant Human Apoptotic protease-activating factor 1 protein (104-415AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:50-1:200, IF:1:50-1:500 |
Примечание | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cancer, Cell biology, Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA001885LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA001885LA01HU at dilution of 1:100
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Immunofluorescence staining of A549 cells with CSB-PA001885LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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