Объем | 100 мкг |
Синонимы | Aquaporin-1 (AQP-1) (Aquaporin-CHIP) (Urine water channel) (Water channel protein for red blood cells and kidney proximal tubule), AQP1, CHIP28 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P29972 |
Иммуноген | Recombinant Human Aquaporin-1 protein (220-269AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
Примечание | Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cancer, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: AQP1 antibody at 16µg/ml
Lane 1: Rat heart tissue
Lane 2: Mouse skeletal muscle tissue
Lane 3: A549 whole cell lysate
Lane 4: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29, 20, 24, 17 kDa
Observed band size: 29 kDa
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Immunofluorescent analysis of HepG2 cells using CSB-PA16085A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating AQP1 in Mouse skeletal muscle tissue
Lane 1: Rabbit control IgG instead of CSB-PA16085A0Rb in Mouse skeletal muscle tissue.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA16085A0Rb (8µg) + Mouse skeletal muscle tissue (500µg)
Lane 3: Mouse skeletal muscle tissue (10µg)
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