| Объем | 50 мкг |
| Синонимы | Annexin A2 (Annexin II) (Annexin-2) (Calpactin I heavy chain) (Calpactin-1 heavy chain) (Chromobindin-8) (Lipocortin II) (Placental anticoagulant protein IV) (PAP-IV) (Protein I) (p36), ANXA2, ANX2 ANX2L4 CAL1H LPC2D |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P07355 |
| Иммуноген | Recombinant Human Annexin A2 protein (2-339AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, U251 whole cell lysate, Mouse lung tissue, Mouse liver tissue, Mouse kidney tissue
All lanes: ANXA2 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 41 kDa
Observed band size: 39 kDa
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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA001840HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA001840HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA001840HA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA001840HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating ANXA2 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA001840HA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA001840HA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Western Blot
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate
All lanes: ANXA2 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 41 kDa
Observed band size: 39 kDa
 |
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA001840HA01HU at dilution of 1:100
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Наименование: ANXA2 Antibody.