| Объем | 50 мкл |
| Синонимы | Apolipoprotein A-II, Apo-AII, ApoA-II, Apolipoprotein A2, Proapolipoprotein A-II, ProapoA-II, Truncated apolipoprotein A-II, Apolipoprotein A-II(1-76), APOA2 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P02652 |
| Иммуноген | A synthesized peptide derived from human APOA2 |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, FC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Cardiovascular |
| Аббревиатура | Apolipoprotein A-II |
| Примечание | May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: 293T whole cell lysate, A549 whole cell lysate
All lanes: Apolipoprotein A II antibody at 0.87µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 KDa
Observed band size: 12 KDa
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IHC image of CSB-RA001915A0HU diluted at 1:87.5 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Overlay histogram showing A549 cells stained with CSB-RA001915A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Наименование: APOA2 Antibody.