Объем | 100 мкл |
Синонимы | autophagy related 5 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9H1Y0 |
Иммуноген | Fusion protein of human ATG5 |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC,ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
Примечание | Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen affinity purification |
Области исследований | Cancer,Cardiovascular,Cell biology,Metabolism,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA028037(ATG5 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ?200)
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA028037(ATG5 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ?200)
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Gel: 12%SDS-PAGE, Lysate: 40 ?g, Lane 1-2: NIH/3T3 cells, A172 cells, Primary antibody: CSB-PA028037(ATG5 Antibody) at dilution 1/475, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
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