| Объем | 50 мкл |
| Синонимы | ATP synthase subunit beta, mitochondrial (EC 3.6.3.14), ATP5B, ATPMB ATPSB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P06576 |
| Иммуноген | Recombinant Human ATP synthase subunit beta, mitochondrial protein (230-529AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200 |
| Примечание | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATP synthase subunit beta, mitochondrial antibody at 9µg/ml
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA002350ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA002350ESR2HU at dilution of 1:100
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Наименование: ATP5B Antibody.