Объем | 100 мкг |
Синонимы | Autophagy-related protein 16-1 (APG16-like 1), ATG16L1, APG16L |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q676U5 |
Иммуноген | Recombinant Human Autophagy-related protein 16-1 protein (1-607AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:20-1:200, IF:1:200-1:500 |
Примечание | Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Neuroscience, Cancer, Cardiovascular, Cell biology, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA734569LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA734569LA01HU at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA734569LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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