| Объем | 100 мкг |
| Синонимы | Cysteine protease ATG4B (EC 3.4.22.-) (AUT-like 1 cysteine endopeptidase) (Autophagin-1) (Autophagy-related cysteine endopeptidase 1) (Autophagy-related protein 4 homolog B) (hAPG4B), ATG4B, APG4B AUTL1 KIAA0943 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9Y4P1 |
| Иммуноген | Recombinant Human Cysteine protease ATG4B protein (350-400AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Cysteine protease ATG4B |
| Области исследований | Cancer, Cardiovascular, Cell biology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: ATG4B antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45, 53, 32, 38, 43 kDa
Observed band size: 45 kDa
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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA002290LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA002290LA01HU at dilution of 1:100
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Immunofluorescent analysis of A549 cells using CSB-PA002290LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: ATG4B Antibody.