| Объем | 100 мкг |
| Синонимы | Acid-sensing ion channel 1 (ASIC1) (Amiloride-sensitive cation channel 2, neuronal) (Brain sodium channel 2) (BNaC2), ASIC1, ACCN2 BNAC2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P78348 |
| Иммуноген | Recombinant Human Acid-sensing ion channel 1 protein (195-297AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
| Примечание | Isoform 2 and isoform 3 function as proton-gated sodium channels, they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Acid-sensing ion channel 1 |
| Области исследований | Neuroscience |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: ASIC1 antibody at 4.24µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 65, 63 kDa
Observed band size: 60 kDa
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Immunofluorescence staining of HepG2 cells with CSB-PA001142LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: ASIC1 Antibody.