| Объем | 50 мкг |
| Синонимы | Aspartyl/asparaginyl beta-hydroxylase (EC 1.14.11.16) (Aspartate beta-hydroxylase) (ASP beta-hydroxylase) (Peptide-aspartate beta-dioxygenase), ASPH, BAH |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q12797 |
| Иммуноген | Recombinant Human Aspartyl/asparaginyl beta-hydroxylase protein (75-270AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Isoform 1:specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. Ref.13 Isoform 8:membrane-bound Ca2+-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Aspartyl/asparaginyl beta-hydroxylase antibody at 2µg/ml
Lane 1: Mouse stomach tissue
Lane 2: jurkat whole cell lysate
Lane 3: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29 kDa
Observed band size: 86 kDa
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Western blot
All lanes: ASPH antibody at 4 µg/ml
Lane 1: Mouse lung tissue
Lane 2: Mouse kidney tissue
Lane 3: Mouse brain tissue
Lane 4: Mouse thymus tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29 kDa
Observed band size: 86 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002226HA01HU at dilution of 1:100
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IHC image of CSB-PA002226HA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA002226HA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA002226HA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: ASPH Antibody.